Plant Biotechnology Vol.27 No.2
Original Papers
Isolation and molecular characterization of a SoWRKY1 transcription factor from spinach (Spinacia oleracea)
Shengzhu Huang, Hiroyuki Yamamoto, Nobuyuki Ide, Shinji Mizuno, Naomasa Shiraishi, Takahide Sato, Hiroki Nakagawa, Masatoshi Sonoda. . . . . . . 121 [PDF]
Using a yeast one-hybrid system we isolated SoWRKY1 from spinach and characterized its function. This study suggested that SoWRKY1 might be involved as a transcription factor in defense-related signal transduction pathways of spinach.
Characterization of MYB proteins acting as transcriptional regulatory factors for carrot phenylalanine ammonia-lyase gene (DcPAL3)
Tatsuya Wako, Soichi Kimura, Yukie Chikagawa, Yoshihiro Ozeki. . . . . . . 131 [PDF]
Box-L in phenylalanine ammonia-lyase gene (DcPAL3) was the regulatory cis-element and five DcMYB cDNAs were isolated for the candidates as binding to box-L from anthocyanin-synthesizing carrot cultured cells. It was suggested that two of five DcMYBs played an important role in regulation of DcPAL3 expression.
Expression of gene for Dioscorea batatas tuber lectin 1 in transgenic tobacco confers resistance to green-peach aphid
Tetsuya Kato, Masatoshi Hori, Tomohisa Ogawa, Koji Muramoto, Kinya Toriyama. . . . . . . 141 [PDF]
Transgenic tobacco expressing cDNA for Dioscorea batatas tuber lectin 1(DB1) under the control of Cauliflower mosaic virus 35S promoter or phloem-specific promoter of sucrose synthase-1 gene reduced the number of survival aphids to 60%, indicating that DB1 can be used to enhance resistance to sap-sucking insects in transgenic crops.
Generation of phenotypically normal marker-free transgenic plants of Kalanchoe blossfeldiana through hairy root induction
Gunaratnam Thirukkumarana, Valentine Otang Ntui, Raham Sher Khan, Ikuo Nakamura, Masahiro Mii. . . . . . . 147 [PDF]
Marker-free transgenic plants with normal phenotype were successfully obtained in Kalanchoe blossfeldiana, an important ornamental crop, through the hairy root and abnormal shoot formation with Ri-syndrome by using Agrobacterium-mediated transformation method with rol-type MAT vector, pMAT101.
Morphological variation in Tricyrtis hirta plants regenerated from heavy ion beam-irradiated embryogenic calluses
Masaru Nakano, Junji Amano, Yusuke Watanabe, Toshikazu Nomizu, Mami Suzuki, Keiko Mizunashi, Shiro Mori, Sachiko Kuwayama, Dong-Sheng Han, Hiroyuki Saito, Hiromichi Ryuto, Nobuhisa Fukunishi, Tomoko Abe. . . . . . . 155 [PDF]
In order to induce horticulturally valuable mutants in Tricyrtis hirta, embryogenic calluses of this species were irradiated with 12C+6 ion beams. Several attractive variations such as dwarfism, slender and deep green leaves, and large flowers were observed in plants regenerated from irradiated calluses.
Transcriptomic response of Arabidopsis thaliana to tunicamycin-induced endoplasmic reticulum stress
Yuji Iwata, Masayo Sakiyama, Mi-Hyun Lee, Nozomu Koizumi. . . . . . . 161 [PDF] [Supplement]
Transcriptome analysis of the ER stress response was conducted in Arabidopsis using tunicamycin. 259 tunicamycin-responsive genes were classified into six distinct expression classes. Bioinformatic analysis identified a potential novel cis-element. Considerable number of tunicamycin-inducible genes is coordinately up-regulated at a late pollen development stage.
Establishment of an Agrobacterium-mediated transformation system for Periploca sepium Bunge
Ren Chen, Mayumi Gyokusen, Yoshihisa Nakazawa, Yinquan Su, Koichiro Gyokusen. . . . . . . 173 [PDF]
By investigating the important factors (Agrobacterium strain, co-cultivation treatment, and antibiotic selection regime) that affected the transformation efficiency, a rapid and efficient system for a cis-polyisoprene-producing plant, Periploca sepium Bunge transformation was established.
Ectopic expression of an AP3-like and a PI-like genes from 'Sekkoku' orchid (Dendrobium moniliforme) causes the homeotic conversion of sepals to petals in whorl 1 and the suppression of carpel development in whorl 4 in Arabidopsis flowers
Supatida Sirisawat, Hiroshi Ezura, Naoya Fukuda, Takahiro Kounosu, Takashi Handa. . . . . . . 183 [PDF]
Three class-B MADS-box genes were isolated from Dendrobium moniliforme. Two of 3 genes were expressed in all four floral whorls in the orchid, whereas another one was expressed only in whorls 2, 3, and 4. The ectopic expression of 2 genes in Arabidpsis transformants caused a sepal-to-petal conversion in whorl 1.
Short Communications
Accumulation of lipid peroxide-derived, toxicα, β-unsaturated aldehydes (E)-2-pentenal, acrolein and (E)-2-hexenal in leaves under photoinhibitory illumination
Jun'ichi Mano, Kenji Tokushige, Hirotoshi Mizoguchi, Hiroyuki Fujii, Sergey Khorobrykh. . . . . . . 193 [PDF] [Supplement]
Overexpression of 2-alkenal reductase (AER) in tobacco confers tolerance against oxidative stress. Here we report that (E)-2-pentenal, acrolein and (E)-2-hexenal were increased in leaves on storng illumination more in the wild type tobacco than in the AER-overexpressing lines. The phototolerance of the AER-overexpressing line can be explained by the detoxification of these 2-alkenals.
Gene Note
An aromatic prenyltransferase-like gene HlPT-1 preferentially expressed in lupulin glands of hop
Yusuke Tsurumaru, Kanako Sasaki, Tatsuya Miyawaki, Takayuki Momma, Naoyuki Umemoto, Kazufumi Yazaki. . . . . . . 199 [PDF]
A cDNA encoding a putative aromatic prenyltransferase was found in ca. 11,000 EST clones of hop (Humulus lupulus). This gene, HlPT-1, was preferentially expressed in hop corns (female flowers), especially in the lupulin glands. GFP fusion protein analysis revealed that the gene product is localized in planstds.
Metabolomics Note
Transient increase in salicylic acid and its glucose conjugates after wounding in Arabidopsis leaves
Takumi Ogawa, Takeshi Ara, Koh Aoki, Hideyuki Suzuki, Daisuke Shibata. . . . . . . 205 [PDF]
We analyzed metabolic changes after mechanical wounding in Arabidopsis leaves by liquid chromatography time-of-flight mass spectrometry, and found activation of salicylic acid metabolism after wounding, suggesting a role of salicylic acid in wound healing.
Tissue Culture Note
In vitro propagation of pecan [Carya illinoinensis (Wangenh) K. Koch]
Nilima N. Renukdas, Muthusamy Manoharan, James O. Garner, Jr.. . . . . . . 211 [PDF]
An efficient method for in vitro propagation has been developed for pecan [Carya illinoinensis (Wangenh) K. Koch], a highly recalcitrant nut species, through nodal explants. The regenerated multiple shoots were successfully rooted and the plantlets established in the greenhouse. This is a simple and efficient protocol that may be used to propagate pecan plants through micropropagation.
Transgenic Note
Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean
Tetsuya Yamada, Satoshi Watanabe, Maiko Arai, Kyuya Harada, Keisuke Kitamura. . . . . . . 217 [PDF]
We improved a protocol of Agrobacterium-mediated transformation in soybean using the cotyledonary node that was wounded with a micro-brush. The transformation frequency in the method presented here was 4 times as high as in that of our previous method.